Journal: Scientific Reports

Article Title: Insight into the cellular fate and toxicity of aluminium adjuvants used in clinically approved human vaccinations

doi: 10.1038/srep31578

Figure Lengend Snippet: Representative lumogallion staining of agar-paraffin embedded (2 μm sections) THP-1 cells co-cultured with ( a ) 2.5, ( b ) 25.0, ( c ) 50.0 or ( d ) 100.0 μg/mL Alhydrogel ® (Brenntag Biosector, Denmark). Cell sections were incubated for 24 h in 100 μM lumogallion, 50 mM PIPES, pH 7.4. Slides were mounted with ProLong ® Gold Antifade Reagent with DAPI. All images depict lumogallion staining (orange) overlaid with DAPI-staining (blue). Magnified inserts show close-ups of individual cells with the light channel overlaid. White arrows highlight both individual and distinguishable adjuvant particles. Magnification X 1000, scale bars: 20 μm.

Article Snippet: Sections were finally air dried and mounted using ProLong ® Gold Antifade Reagent with 4′,6-diamidino-2-phenylindole, dihydrochloride (DAPI) (Life Technologies, UK) prior to storing horizontally for 24 h at 4 °C.

Techniques: Staining, Cell Culture, Incubation, Adjuvant

Journal: Scientific Reports

Article Title: Insight into the cellular fate and toxicity of aluminium adjuvants used in clinically approved human vaccinations

doi: 10.1038/srep31578

Figure Lengend Snippet: Representative lumogallion staining of agar-paraffin embedded (2 μm sections) THP-1 cells co-cultured with ( a ) 2.5, ( b ) 25, ( c ) 50 or ( d ) 100 μg/mL Adju-Phos ® (Brenntag Biosector, Denmark). Cell sections were incubated for 24 h in 100 μM lumogallion, 50 mM PIPES, pH 7.4. Slides were mounted with ProLong ® Gold Antifade Reagent with DAPI. All images depict lumogallion staining (orange) overlaid with DAPI-staining (blue). Magnified inserts show close-ups of individual cells with the light channel overlaid. White arrows highlight both individual and distinguishable adjuvant particles. Magnification X 1000, scale bars: 20 μm.

Article Snippet: Sections were finally air dried and mounted using ProLong ® Gold Antifade Reagent with 4′,6-diamidino-2-phenylindole, dihydrochloride (DAPI) (Life Technologies, UK) prior to storing horizontally for 24 h at 4 °C.

Techniques: Staining, Cell Culture, Incubation, Adjuvant

Journal: Scientific Reports

Article Title: Insight into the cellular fate and toxicity of aluminium adjuvants used in clinically approved human vaccinations

doi: 10.1038/srep31578

Figure Lengend Snippet: Representative lumogallion staining of agar-paraffin embedded (2 μm sections) THP-1 cells co-cultured with ( a ) 2.5, ( b ) 25, ( c ) 50 or ( d ) 100 μg/mL Imject™ Alum (Thermo Scientific). Cell sections were incubated for 24 h in 100 μM lumogallion, 50 mM PIPES, pH 7.4. Slides were mounted with ProLong ® Gold Antifade Reagent with DAPI. All images depict lumogallion staining (orange) overlaid with DAPI-staining (blue). Magnified inserts show close-ups of individual cells with the light channel overlaid. White arrows highlight both individual and distinguishable adjuvant particles. Magnification X 1000, scale bars: 20 μm.

Article Snippet: Sections were finally air dried and mounted using ProLong ® Gold Antifade Reagent with 4′,6-diamidino-2-phenylindole, dihydrochloride (DAPI) (Life Technologies, UK) prior to storing horizontally for 24 h at 4 °C.

Techniques: Staining, Cell Culture, Incubation, Adjuvant

Journal: Oncotarget

Article Title: Silencing of casein kinase 1 delta reduces migration and metastasis of triple negative breast cancer cells

doi: 10.18632/oncotarget.25738

Figure Lengend Snippet: ( A ) Scatter plot of gene expression analysis by RT 2 Profiler PCR Array in the shNT MDA-MB-231 cells compared to shCSNK1D cells. The central line indicates unchanged gene expression. ( B ) Taqman validation of genes identified in the RT Profiler array. The histogram represents fold change of genes up-or down-regulated in shCSNK1D compared to shNT control cells. ( C ) Western blot analysis of the expression of occludin (OCLN), junction adhesion molecule A (F11R) and claudin-1 (CLDN-1) in control (shNT) and inactivated cell lines (shCSNK1D). ( D ) Detection of OCLN and F11R by immunofluorescence in shCSNK1D compared to control shNT cells. Nuclei were stained with Dapi. Arrows indicate the increased expression of tight junction proteins.

Article Snippet: After washing, the cells were mounted with Prolong gold antifade medium with DAPI (Cell Signaling).

Techniques: Gene Expression, Biomarker Discovery, Control, Western Blot, Expressing, Immunofluorescence, Staining